Multichannel LED light Source BBO-Niji - BlueBox Optics
-Fluorescence microscopy applications-
LOVE ITS MODULAR DESIGN, PRICE, FLEXIBILITY AND SOFTWARE INTEGRATION!Advantages of BBO-Niji multichannel LED light source:
- Full system with 7+1 LED channels, controllable independently or simultaneously
- UV-VIS-NIR spectral coverage with high powers over that of Mercury, metal halide and Xenon lamps
- Modular design (purchase only the channels you need now and upgrade with the rest of channels later on by yourself)
- "Constant current" and "constant optical power" illumination modes selectable, according to your experiment
- Excitation filters selectable for each channel and LED channels
- Compatible with the most common microscopes such us Nikon, Leica, Olympus, Zeiss
- Compatible with the most common third-party softwares: Metamorph, Micromanager, NIS Elements…
- Very long lifetime
- Niji LED light source completely free of Mercury (safe and sustainable, lower environmental cost and ownership cost)
- Cost-effective
Light Output specifications of BBO-Niji multichannel LED light source
- | UV | Royal Blue | Blue | Cyan | Green/ Yellow | Red | Far Red |
Wavelength (nm) | 395 | 445 | 470 | 505 | 556 | 635 | 725 |
Bandwidth FWHM (nm) | 10 | 18 | 19 | 31 | 62 | 18 | 38 |
Typ. Optical Power (mW)* | 120 | 200 | 185 | 90 | 550 | 225 | 70 |
Recommended Filter Sets | LED-DAPI-A-000 | LED-CFP-A-000 | LED-FITC-A-000 | LED-YFP-A-000 | LED-TRITC-A-000 LED-mCherry-A-000 | LED-Cy5-A-000 | LED-Cy7-A/B-000 |
Note: Optical output powers are given for a ø3 mm liquid light guide, use of a ø5 mm light guide will provide approximately 50% more power. Wavelengths and powers are approximate and may vary on LED batch. Each niji will be supplied with full optical power and wavelength test data.
*Typ. Optical Power is specified for the complete system. By removing the channels you do not need, you will reach higher output channels in the wavelengths of interest. Please, tell us your power requirements.
Software of BBO-Niji multichannel LED light source:
- The GUI (Graphical User Interface) is very intuitive and user-friendly allowing to control individually or simultaneously the channel (power and On/off). XML files can be uploaded and different experimental parameter configuration can be saved.
- Niji allows the integration in most of the microscope softwares
- Niji has available the drivers to operate with Micromanager, Micromanager 2 and now a fully compatible Metamorph driver.
Features of BBO-Niji multichannel LED light source:
Modular Channels | Up to seven channels – user fit* | Operation | Control via dedicated software, MicroManager, Metamorph tablet, or third party software |
Sources | Solid state LED | Programmable Commands | XML upload for complete flexibility |
Wavelength Range | 380 - 730 nm available** | Optical Safety | Key switch, light guide, and lid-off interlock |
Noise Level | Approx. 47 dB | Dimensions | 315 x 288 x 107 mm |
Bandpass Filters | Individual bandpass filter holders for each channel | Weight | 4.7 kg |
Light Interface | Liquid light guide with microscope collimators, available separately | Warranty | 12 months, optional 3 years |
Connection | USB -A (powered), USB-B, RS232 (serial), and TTL via a 15-way connector | | |
*The niji modular chassis allows user upgrades
** New high power 390 nm UV channel is now coming
Applications for BBO-Niji multichannel LED light source:
Almost every application where gas lamps (high pressure mercury vapor arc-discharge lamps-HBO, metal halide lamps...) are used until now:
Widefield microscopy | Life cell imaging (allows low power illumination to avoid photo-bleaching and photo-toxicity | Fluorescence microscopy | Color rendering | TIRF microscopy | Multi-photon microscopy | SIM microscopy (together with a spatial light modulator) | For using with fixed immunofluorescence samples and CLEM | Integration in slide scanning systems | Widefield Optogenetics | In Vitro Optogenetics | Control of horse muscle | Low cost systems (for OEM integration) | Excitation of DAPI (choose the high power 390 nm UV version)
Short tutorial about "how to use the Bluebox Optics Niji with Micromanager" (clik here)
Download the white paper (WP) in the tap "Descargar documentación" for learning about the envaironmental and economical saving when you replace your Halogen and Metal halide arc lamps for light sources based on LED technology, like the BBO-Niji (Bluebox).
Also, you may need the following products:
- sCMOS front and back-illuminated cameras (PCO)- Optical filters (fluorescence filter sets, dichroics, bandpass filters..)- Micromanipulators- Optical tables and breadboards
